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Microbial lipases : at the interface of aqueous and non-aqueous media: a review

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posted on 2008-09-01, 00:00 authored by Madan Verma, W Azmi, S Kanwar
In recent times, biotechnological applications of microbial lipases in synthesis of many organic molecules have rapidly increased in non-aqueous media. Microbial lipases are the working horses' in biocatalysis and have been extensively studied when their exceptionally high stability in non-aqueous media has been discovered. Stability of lipases in organic solvents makes them commercially feasibile in the enzymatic esterification reactions. Their stability is affected by temperature, reaction medium, water concentration and by the biocatalyst's preparation. An optimization process for ester synthesis from pilot scale to industrial scale in the reaction medium is discussed. The water released during the esterification process can be controlled over a wide range and has a profound effect on the activity of the lipases. Approaches to lipase catalysis like protein engineering, directed evolution and metagenome approach were studied. This review reports the recent development in the field of non-aqueous microbial lipase catalysis and factors controlling the esterification/transesterification processes in organic media.

History

Journal

Acta microbiologica et immunologica Hungarica

Volume

55

Pagination

265 - 294

Location

Budapest, Hungary

Open access

  • Yes

ISSN

1217-8950

eISSN

1588-2640

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2006, Akadémiai Kiadó

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