Deakin University
Browse

File(s) not publicly available

Microfluidic array chip for parallel detection of waterborne bacteria

Version 3 2024-06-19, 14:11
Version 2 2024-06-05, 00:39
Version 1 2022-09-30, 01:59
journal contribution
posted on 2024-06-19, 14:11 authored by L Gorgannezhad, KR Sreejith, Jun ZhangJun Zhang, G Kijanka, M Christie, H Stratton, NT Nguyen
The polymerase chain reaction (PCR) is a robust technique used to make multiple copies of a segment of DNA. However, the available PCR platforms require elaborate and time-consuming operations or costly instruments, hindering their application. Herein, we introduce a sandwiched glass–polydimethylsiloxane (PDMS)–glass microchip containing an array of reactors for the real-time PCR-based detection of multiple waterborne bacteria. The PCR solution was loaded into the array of reactors in a single step utilising capillary filling, eliminating the need for pumps, valves, and liquid handling instruments. Issues of generating and trapping bubbles during the loading chip step were addressed by creating smooth internal reactor surfaces. Triton X-100 was used to enhance PCR compatibility in the chip by minimising the nonspecific adsorption of enzymes. A custom-made real-time PCR instrument was also fabricated to provide thermal cycling to the array chip. The microfluidic device was successfully demonstrated for microbial faecal source tracking (MST) in water.

History

Journal

Micromachines

Volume

10

Pagination

883-883

Location

Switzerland

ISSN

2072-666X

eISSN

2072-666X

Language

en

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

12

Publisher

MDPI AG

Usage metrics

    Research Publications

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC