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Microparticles: major transport vehicles for distinct microRNAs in circulation

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Version 2 2024-06-04, 15:42
Version 1 2018-08-24, 14:25
journal contribution
posted on 2024-06-04, 15:42 authored by Philipp Diehl, Alba Fricke, Laura Sander, Johannes Stamm, Nicole Bassler, Nay Htun, Mark ZiemannMark Ziemann, Thomas Helbing, Assam El-Osta, Jeremy BM Jowett, Karlheinz Peter
AIMS: Circulating microRNAs (miRNAs) have attracted major interest as biomarkers for cardiovascular diseases. Since RNases are abundant in circulating blood, there needs to be a mechanism protecting miRNAs from degradation. We hypothesized that microparticles (MP) represent protective transport vehicles for miRNAs and that these are specifically packaged by their maternal cells. METHODS AND RESULTS: Conventional plasma preparations, such as the ones used for biomarker detection, are shown to contain substantial numbers of platelet-, leucocyte-, and endothelial cell-derived MP. To analyse the widest spectrum of miRNAs, Next Generation Sequencing was used to assess miRNA profiles of MP and their corresponding stimulated and non-stimulated cells of origin. THP-1 (monocytic origin) and human umbilical vein endothelial cell (HUVEC) MP were used for representing circulating MP at a high purity. miRNA profiles of MP differed significantly from those of stimulated and non-stimulated maternal THP-1 cells and HUVECs, respectively. Quantitative reverse transcription-polymerase chain reaction of miRNAs which have been associated with cardiovascular diseases also demonstrated significant differences in miRNA profiles between platelets and their MP. Notably, the main fraction of miRNA in plasma was localized in MP. Furthermore, miRNA profiles of MP differed significantly between patients with stable and unstable coronary artery disease. CONCLUSION: Circulating MP represent transport vehicles for large numbers of specific miRNAs, which have been associated with cardiovascular diseases. miRNA profiles of MP are significantly different from their maternal cells, indicating an active mechanism of selective 'packaging' from cells into MP. These findings describe an interesting mechanism for transferring gene-regulatory function from MP-releasing cells to target cells via MP circulating in blood.

History

Journal

Cardiovascular research

Volume

93

Pagination

633-644

Location

Oxford, Eng.

Open access

  • Yes

eISSN

1755-3245

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2012, The Author

Issue

4

Publisher

Oxford University Press