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Microvascular recruitment is an early insulin effect that regulates skeletal muscle glucose uptake in vivo

Version 2 2024-06-04, 12:42
Version 1 2017-03-22, 16:52
journal contribution
posted on 2004-06-01, 00:00 authored by Michelle KeskeMichelle Keske, L H Clerk, J R Lindner, A L Klibanov, M G Clark, S Rattigan, E J Barrett
Insulin increases glucose disposal into muscle. In addition, in vivo insulin elicits distinct nitric oxide synthase-dependent vascular responses to increase total skeletal muscle blood flow and to recruit muscle capillaries (by relaxing resistance and terminal arterioles, respectively). In the current study, we compared the temporal sequence of vascular and metabolic responses to a 30-min physiological infusion of insulin (3 mU. min(-1). kg(-1), euglycemic clamp) or saline in rat skeletal muscle in vivo. We used contrast-enhanced ultrasound to continuously quantify microvascular volume. Insulin recruited microvasculature within 5-10 min (P < 0.05), and this preceded both activation of insulin-signaling pathways and increases in glucose disposal in muscle, as well as changes in total leg blood flow. Moreover, l-NAME (N(omega)-nitro-l-arginine-methyl ester), a specific inhibitor of nitric oxide synthase, blocked this early microvascular recruitment (P < 0.05) and at least partially inhibited early increases in muscle glucose uptake (P < 0.05). We conclude that insulin rapidly recruits skeletal muscle capillaries in vivo by a nitric oxide-dependent action, and the increase in capillary recruitment may contribute to the subsequent glucose uptake.

History

Journal

Diabetes

Volume

53

Issue

6

Pagination

1418 - 1423

Publisher

American Diabetes Association

Location

Arlington, Va.

ISSN

0012-1797

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2004, American Diabetes Association