haswell-miminuminhibitory-2012.pdf (511.37 kB)
Minimum inhibitory and bacterial concentrations of theaflavin and synergistic combinations with epicatechin and quercetin against clinical isolates of Stenotrophomonas Maltophilia
journal contribution
posted on 2012-05-01, 00:00 authored by J Betts, C Murphy, S Kelly, Stephen HaswellStenotrophomonas maltophilia is an important nosocomial pathogen with intrinsic resistance to multiple antibiotics. Previous investigations have shown flavanols from black tea to possess antibacterial activity. This study describes the determination of minimum inhibitory concentrations and minimum bactericidal concentration for theaflavin independently and in formulations with the polyphenols epicatechin and quercetin against nine clinical isolates of Stenotrophomonas maltophilia and the control isolate NCTC 130141 via the microtitre assay. The results demonstrate that theaflavin has strong antibacterial activity and also shows significant synergism with epicatechin and quercetin. The minimum inhibitory concentrations of the isolates range between 200-400 g/mL for theaflavin and 100-200 g/mL for both theaflavin:epicatechin and theaflavin:quercetin combinations. The minimum bactericidal concentrations were discovered to be a 2 fold increase on those of the minimum inhibitory concentrations. The research highlights the potential use of polyphenols for the clinical treatment of highly antibiotic resistant bacteria.
History
Journal
Journal of microbiology, biotechnology and food sciencesVolume
1Issue
5Pagination
1250 - 1258Publisher
Slovak University of Agriculture in NitraLocation
Nitra, SlovakiaISSN
1338-5178Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2012, Slovak University of Agriculture in NitraUsage metrics
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