Deakin University
Browse

File(s) not publicly available

Molecular characterization and in-vitro hormonal requirements for expression of two casein genes from a marsupial

Version 2 2024-06-13, 14:55
Version 1 2021-11-22, 08:34
journal contribution
posted on 2024-06-13, 14:55 authored by C Collet, R Joseph, K Nicholas
ABSTRACT Two marsupial casein genes have been isolated from a tammar wallaby (Macropus eugenii) mammary gland cDNA library. Comparisons of the tammar α- and β-casein genes with their eutherian homologues reveal extensive divergence at the levels of nucleotide and amino acid sequences. Regions of similarity between the tammar and eutherian Ca2+-sensitive caseins are restricted to the major phosphorylation sites and the signal peptides. Quantification of casein mRNA levels in hormone-stimulated mammary gland explants from tammars in late pregnancy suggests that maximal induction of the β-casein gene is dependent upon prolactin and insulin, while maximal induction of the α-casein gene is dependent upon prolactin, insulin and cortisol. These results are in contrast to earlier studies which show that the maximal induction of a putative 19 kDa casein, α-lactalbumin and β-lactoglobulin in the tammar mammary gland is dependent upon prolactin alone. The expression of the latter three genes is not modulated by other hormones known to play a role in the in-vitro initiation of lactation in eutherians.

History

Journal

Journal of Molecular Endocrinology

Volume

8

Pagination

13-20

Location

England

ISSN

0952-5041

eISSN

1479-6813

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

1

Publisher

Bioscientifica