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Molecular decoration of ceramic supports for highly effective enzyme immobilization-material approach

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Version 2 2024-06-13, 17:36
Version 1 2022-05-31, 09:16
journal contribution
posted on 2024-06-13, 17:36 authored by J Kujawa, M Głodek, I Koter, B Ośmialowski, K Knozowska, S Al-Gharabli, LF Dumée, W Kujawski
A highly effective method was developed to functionalize ceramic supports (Al2O3 powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically evaluate the impact of various spacer molecules grafted onto the alumina materials will affect both the immobilization of the enzymes and specific material surface properties, critical to enzymatic reactors performance. The enzyme loading was significantly improved for the supports modified with shorter spacer molecules, which possessed higher grafting effectiveness on the order of 90%. The specific enzyme activity was found to be much higher for samples functionalized with longer modifiers yielding excellent enantioselectivity >97%. However, the enantiomeric ratio of the immobilized lipase was slightly lower in the case of shorter spacer molecules.

History

Journal

Materials

Volume

14

Pagination

1-18

Location

Basel, Switzerland

eISSN

1996-1944

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

1

Publisher

MDPI / MDPI AG (Multidisciplinary Digital Publishing Institute)

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