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Molecular identification of marine yeast and its spectroscopic analysis establishes unsaturated fatty acid accumulation

journal contribution
posted on 2012-10-01, 00:00 authored by A Gupta, Jitraporn Vongsvivut, Colin BarrowColin Barrow, Munish PuriMunish Puri
Marine microbes are competent organisms, some of which can accumulate large amounts of lipids. A yeast strain, Rhodotorula mucilaginosa AMCQ8A was isolated from the marine water of the Queenscliff region, Victoria, Australia. The yeast isolate was identified by sequencing 18s rDNA genes. Scanning electron microscopy images revealed scars on the surface of the yeast cells. The Fourier transform infrared spectroscopy microspectroscopy studies demonstrated the presence of unsaturated fatty acids by differential microscopic analysis. The sharp band at 1745 cm-1 was represented by ν(C=O) stretches of ester functional groups from lipids and fats, and therefore indicated the presence of total lipids produced by the cells. Over 65% of the fatty acids from the yeast strain were analyzed as C16 and C18:1 with omega-3 content from about 6% to 7%. Thus, this marine-derived yeast could be a potential source of lipids, including omega-3 fatty acids. 2012, The Society for Biotechnology, Japan. All rights reserved.

History

Journal

Journal of bioscience and bioengineering

Volume

114

Issue

4

Pagination

411 - 417

Publisher

Elsevier BV

Location

Amsterdam, The Netherlands

ISSN

1347-4421

Language

eng

Notes

Available online 20 June 2012

Publication classification

C1 Refereed article in a scholarly journal; C Journal article

Copyright notice

2012, Elsevier