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Multifunctional nanoparticle-EpCAM aptamer bioconjugates: a paradigm for targeted drug delivery and imaging in cancer therapy
The promising proposition of multifunctional nanoparticles for cancer diagnostics and therapeutics has inspired the development of theranostic approach for improved cancer therapy. Moreover, active targeting of drug carrier to specific target site is crucial for providing efficient delivery of therapeutics and imaging agents. In this regard, the present study investigates the theranostic capabilities of nutlin-3a loaded poly (lactide-co-glycolide) nanoparticles, functionalized with a targeting ligand (EpCAM aptamer) and an imaging agent (quantum dots) for cancer therapy and bioimaging. A wide spectrum of in vitro analysis (cellular uptake study, cytotoxicity assay, cell cycle and apoptosis analysis, apoptosis associated proteins study) revealed superior therapeutic potentiality of targeted drug loaded NPs over other formulations in EpCAM expressing cells. Moreover, our nanotheranostic system served as a superlative bio-imaging modality both in 2D monolayer culture and tumor spheroid model. Our result suggests that, these aptamer-guided multifunctional NPs may act as indispensable nanotheranostic approach toward cancer therapy.
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Journal
Nanomedicine : nanotechnology, biology and medicineIssue
In pressPagination
1 - 11Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
eISSN
1549-9642Language
EnglishPublication classification
C1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2015, ElsevierUsage metrics
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No categories selectedKeywords
EpCAM aptamerNanotheranosticsNutlin-3aPLGA NPsQuantum dotsScience & TechnologyLife Sciences & BiomedicineNanoscience & NanotechnologyMedicine, Research & ExperimentalScience & Technology - Other TopicsResearch & Experimental MedicineCELL ADHESION MOLECULEPACLITAXEL-LOADED NANOPARTICLESQUANTUM-DOTLIVE CELLSIN-VITROACTIVATIONFLUORESCENCEAPOPTOSISMARKERCYCLE
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