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Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns
journal contribution
posted on 2015-01-01, 00:00 authored by S Pravadali-Cekic, D Kocic, Xavier ConlanXavier Conlan, R A ShallikerDetector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH• for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.
History
Journal
Journal of liquid chromatography and related technologiesVolume
38Issue
19Pagination
1753 - 1758Publisher
Taylor & FrancisPublisher DOI
ISSN
1082-6076eISSN
1520-572XLanguage
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, Taylor & FrancisUsage metrics
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No categories selectedKeywords
Active flow technologyDPPH center dotHigh performance liquid chromatographyMultiplexed detectionNatural productsParallel segmented flowScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistrySEGMENTED FLOW CHROMATOGRAPHYPERFORMANCE LIQUID-CHROMATOGRAPHYNATURAL-PRODUCTSOUTLET FLOWTECHNOLOGYDERIVATIZATIONSEPARATIONS
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