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Multiplexed dual first-dimension comprehensive two-dimensional gas chromatography-mass spectrometry with contra-directional thermal modulation

journal contribution
posted on 2014-10-24, 00:00 authored by Benjamin Savareear, Matthew R Jacobs, Robert ShellieRobert Shellie
A multiplexed dual-primary column comprehensive two-dimensional gas chromatography-mass spectrometry approach (2GC×GC-MS) is introduced. The approach splits injected samples into two first-dimension columns with different stationary phases, and recombines the two streams into one second-dimension column that terminates at a single detector. The approach produces two two-dimensional chromatograms for each injection, and is made possible by using a dual-stage modulator operated in contra-directional modulation mode. The dual two-dimensional chromatograms produced by this single detector system provide complementary information due to selectivity differences between the three separation columns used in the column ensemble. An aged Australian tea tree (Melaleuca alternifolia) essential oil was analyzed to demonstrate the 2GC×GC-MS approach. The number of compounds separated by each of the GC×GC separations in the 2GC×GC experiment is comparable to conventional GC×GC experiments with matching column configurations. Robust peak assignment was possible for this sample based on the combination of MS library matches and multiple linear retention index searching. Forty-nine components (22 unique) were identified using a non-polar×mid-polar column combination and 34 components (7 unique) were positively identified using a polar×mid-polar column combination. Twenty-seven peak assignments were corroborated by positive identification in both of the multiplexed separations.

History

Journal

Journal of Chromatography A

Volume

1365

Pagination

183 - 190

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

0021-9673

eISSN

1873-3778

Language

eng

Publication classification

C1 Refereed article in a scholarly journal