File(s) under permanent embargo
Natriuretic peptide receptors in the central vasculature of the toad, Bufo marinus
journal contributionposted on 2001-01-01, 00:00 authored by Kirsty Minerds, John DonaldJohn Donald
Natriuretic peptide receptors in the central vasculature of the toad, Bufo marinus, were characterized using autoradiographical, molecular, and physiological techniques. Specific 125I-rat ANP binding sites were present in the carotid and pulmonary arteries, the lateral aorta, the pre- and post-cava, and the jugular vein, and generally occurred in each layer of the blood vessel. The 125I-rat ANP binding was partially displaced by the specific natriuretic peptide receptor C ligand, C-ANF, which indicates the presence of two types of natriuretic peptide receptors in the blood vessels. This was confirmed by a RT-PCR study, which demonstrated that guanylyl cyclase receptor (NPR-GC) and NPR-C mRNAs are expressed in arteries and veins. An in vitro guanylyl cyclase assay showed that frog ANP stimulated the production of cGMP in arterial membrane fractions. Physiological recordings from isolated segments of the carotid and pulmonary arteries and the lateral aorta, which had been pre-constricted with arginine vasotocin, showed that rat ANP, frog ANP and porcine CNP relaxed the vascular smooth muscle with relatively similar potency. Together, the data show that the central vasculature contains two types of natriuretic peptide receptors (NPR-C and NPR-GC) and that the vasculature is a target for ANP and CNP.
JournalComparative biochemistry and physiology - part a: molecular & integrative physiology
Pagination259 - 268
PublisherElsevier Science Inc.
LocationNew York, N. Y.
Publication classificationC1 Refereed article in a scholarly journal
Copyright notice2001, Elsevier Science Inc.
atrial natriuretic peptideblood vesselsclearance receptorsguanylyl cyclasecGMPnatriuretic peptide receptorsc-type natriuretic peptideBufo marinusScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyPhysiologyZoologycGMP, clearance receptorsSMOOTH-MUSCLEBLOOD-VESSELSHUMAN ARTERYB-TYPEATRIALHEARTIDENTIFICATIONBRAINVEINEELPhysiologyZoology