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New DNA modification strategies involving oxime formation

journal contribution
posted on 2024-02-09, 02:02 authored by B Cebon, JN Lambert, D Leung, H Mackie, KL McCluskey, Xuan NguyenXuan Nguyen, C Tassone
A new method for the formation of covalently linked DNA conjugates is described. The method involves installation of a 5´-hydroxylamine nucleophile onto synthetic DNA and condensation with a suitable electrophilic carbonyl compound to form an oxime linkage. Various protection strategies for the hydroxylamine group and their merits are discussed and the formation of an oligonucleotide–peptide conjugate is described.

History

Journal

Australian Journal of Chemistry

Volume

53

Pagination

333-339

Location

Melbourne, VIC.

Publisher DOI

ISSN

0004-9425

eISSN

1445-0038

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

4

Publisher

CSIRO Publishing

Publication URL

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    Research Publications

    Categories

    Keywords

    ANTISENSE OLIGONUCLEOTIDESATTACHMENTChemistryChemistry, MultidisciplinaryconjugateDELIVERYDNAOLIGODEOXYRIBONUCLEOTIDESoligonucleotideoximepeptidephosphoramiditePhysical SciencesScience & Technologysynthesis

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    All Rights Reserved

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