File(s) under permanent embargo
Next-generation metabolic engineering approaches towards development of plant cell suspension cultures as specialized metabolite producing biofactories
journal contributionposted on 2020-12-01, 00:00 authored by Sagar Arya, Jim RookesJim Rookes, David CahillDavid Cahill, Sangram K Lenka
Plant cell suspension culture (PCSC) has emerged as a viable technology to produce plant specialized metabolites (PSM). While Taxol® and ginsenoside are two examples of successfully commercialized PCSC-derived PSM, widespread utilization of the PCSC platform has yet to be realized primarily due to a lack of understanding of the molecular genetics of PSM biosynthesis. Recent advances in computational, molecular and synthetic biology tools provide the opportunity to rapidly characterize and harness the specialized metabolic potential of plants. Here, we discuss the prospects of integrating computational modeling, artificial intelligence, and precision genome editing (CRISPR/Cas and its variants) toolboxes to discover the genetic regulators of PSM. We also explore how synthetic biology can be applied to develop metabolically optimized PSM-producing native and heterologous PCSC systems. Taken together, this review provides an interdisciplinary approach to realize and link the potential of next-generation computational and molecular tools to convert PCSC into commercially viable PSM-producing biofactories.
LocationNew York, N.Y.
Publication classificationC Journal article; C1 Refereed article in a scholarly journal
Copyright notice2020, Elsevier
Read the peer-reviewed publication
Plant cell suspension culturePlant specialized metabolitesPlant gene clustersArtificial gene clusterMetabolic engineeringComputational modelingScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyGENE CLUSTERSTRANSCRIPTOME ANALYSISSECONDARY METABOLITESMETHYL JASMONATENATURAL-PRODUCTSCALLUS-CULTURESDRUG-DELIVERYSYNTHASE GENEBABY-BOOMBIOSYNTHESIS