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No decline in skeletal muscle oxidative capacity with aging in long-term calorically restricted rats: effects are independent of mitochondrial DNA integrity

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journal contribution
posted on 2006-07-01, 00:00 authored by D J Baker, Andrew BetikAndrew Betik, D J Krause, R T Hepple
We investigated if calorie restriction (CR) preserved skeletal muscle oxidative capacity with aging after accounting for life span extension by CR, and determined if mitochondrial content, mitochondrial DNA integrity, and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) were involved. Ad libitum-fed (AL) and CR animals representing young adult, late middle age, and senescence were studied. Whereas citrate synthase and complex IV activities were lower in plantaris and gastrocnemius muscle of young adult CR animals, in contrast to the 15%-40% decline in senescent AL animals, there was no decline with aging in CR animals. There was no decline in citrate synthase protein in gastrocnemius with aging in either group, suggesting that CR preserves oxidative capacity with aging by protecting mitochondrial function rather than content. This protection was independent of mitochondrial DNA damage between groups. However, there was a slower decline in PGC-1α gene expression with aging in CR versus AL animals, suggesting a better maintenance of mitochondrial biogenesis with aging in CR animals. Copyright 2006 by The Gerontological Society of America.

History

Journal

Journals of gerontology - series a biological sciences and medical sciences

Volume

61

Issue

7

Pagination

675 - 684

Publisher

Oxford University Press

Location

Oxford, Eng.

ISSN

1079-5006

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

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