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Novel multifunctional biocompatible gelatin-oleic acid conjugate: self-assembled nanoparticles for drug delivery
journal contribution
posted on 2013-08-01, 00:00 authored by Phuong TranPhuong Tran, Thao Truong-Dinh Tran, Toi Van Vo, Chau Le-Ngoc Vo, Beom-Jin LeeIn this work, a novel, biocompatible conjugates of gelatin and oleic acid (GOC) were synthesized by a novel aqueous solvent-based method that overcame challenges of completely contrary solubility between gelatin and oleic acid (OA). The GO nanoparticles (GONs) and Paclitaxel encapsulated nanoparticles (PTX-GON) were prepared by self-assembly in water. These nanoparticles (NPs) were then conjugated with folic acid (FA) for targeting cervical cancer cells (Hela cells) and were characterized for their various physicochemical and pharmaceutical properties. Fourier transform infrared spectroscopy (FT-IR) and 1H NMR studies indicated the successful synthesis of GOC which showed low critical aggregation concentration in water (0.015 mg/ml). All NPs were stable in human blood serum and their mean diameters were below 300 nm suitable for passive targeting. Powder X-ray diffraction (PXRD) diffractograms showed the reduction in drug crystallinity and hence, leading to the solubility enhancement of PTX. The release of PTX from both PTX-GON and FA conjugated PTX-GON (PTX-FA-GON) was controlled for a long time. The cytotoxicity results demonstrated great advantages of PTX-FA-GON and PTX-GON over the conventional dosage form of pacliaxel (Taxol). These results, therefore, indicate that GOC is a promising material to prepare drug encapsulated NP as a controlled delivery system and PTX-FA-GON is a potential targeted delivery system for cancer therapy.
History
Journal
Journal of biomedical nanotechnologyVolume
9Issue
8Pagination
1416 - 1431Publisher
American Scientific PublishersLocation
Valencia, Calif.Publisher DOI
ISSN
1550-7033Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2013, American Scientific PublishersUsage metrics
Keywords
folic acid (FA)gelatin-oa conjugate (GOC)paclitaxelprotein conjugationself-assembled nanoparticlestargeted drug deliveryScience & TechnologyTechnologyNanoscience & NanotechnologyMaterials Science, BiomaterialsScience & Technology - Other TopicsMaterials ScienceIN-VITROPOLYMERIC NANOPARTICLESPACLITAXEL TAXOL(R)CONTROLLED-RELEASEMICELLESGLYCOLInorganic Chemistry
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