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O-acetylation of sialic acids in N-glycans of Atlantic salmon (Salmo salar) serum is altered by handling stress
journal contribution
posted on 2008-07-01, 00:00 authored by X Liu, Luis AfonsoLuis Afonso, E Altman, S Johnson, L Brown, J LiO-acetylation is one of the major modifications of sialic acids that significantly alters biological properties of the parent molecule. These O-acetylated forms are components of the cellular membrane and can affect physiological and pathological responses. Understanding the role of N-glycans in physiology is of increasing relevance to cellular biologists in various disciplines who study glycoproteomics yet lack information regarding the function of the attached glycans. It is well known that stress may decrease immune function in fish; however, there are only few suitable biomarkers available to monitor the physiological responses under the stress conditions. This study is the first report on the effect of stress on the profile of O-acetylation of sialic acids in fish serum. In order to preserve the relevant structural characteristics as much as possible, native N-glycans were directly analyzed using CE-MS. We have characterized the N-glycans in serum of salmon (Salmo salar) exposed to long-term handling stress (15 s out of the water, daily for 4 wk) and compared with the results obtained from sera of control fish. The results indicated that major N-glycans in salmon serum contained mono-acetylated sialic acids (83%), and that the O-acetylation pattern of sialic acids could be altered by long-term stress.
History
Journal
ProteomicsVolume
8Issue
14Pagination
2849 - 2857Publisher
Wiley - V C H Verlag GmbH & Co. KGaALocation
Weinheim, GermanyPublisher DOI
ISSN
1615-9853eISSN
1615-9861Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2008, Wiley-BlackwellUsage metrics
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No categories selectedKeywords
Atlantic salmonglycomicsglycosylationO-acetylationsialic acidScience & TechnologyLife Sciences & BiomedicineBiochemical Research MethodsBiochemistry & Molecular BiologyLASER-DESORPTION\/IONIZATION-TIMEACUTE LYMPHOBLASTIC-LEUKEMIAFLIGHT MASS-SPECTROMETRYHUMAN COLONIC MUCOSASHORT-TERM9-O-ACETYLATIONTRANSFERRINDERIVATIZATIONPURIFICATION
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