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ORP-3, a human oxysterol-binding protein gene differentially expressed in hematopoietic cells

Version 2 2024-06-16, 13:28
Version 1 2014-10-27, 16:25
journal contribution
posted on 2024-06-16, 13:28 authored by C Gregorio-King, G Collier, J McMillan, C Waugh, J McLeod, Fiona CollierFiona Collier, M Kirkland
Using differential display polymerase chain reaction, a gene was identified in CD34+-enriched populations that had with low or absent expression in CD34- populations. The full coding sequence of this transcript was obtained, and the predicted protein has a high degree of homology to oxysterol-binding protein. This gene has been designated OSBP-related protein 3 (ORP-3). Expression of ORP-3 was found to be 3- to 4-fold higher in CD34+ cells than in CD34- cells. Additionally, expression of this gene was 2-fold higher in the more primitive subfraction of hematopoietic cells defined by the CD34+38- phenotype and was down-regulated with the proliferation and differentiation of CD34+ cells. The ORP-3 predicted protein contains an oxysterol-binding domain. Well-characterized proteins expressing this domain bind oxysterols in a dose-dependent fashion. Biologic activities of oxysterols include inhibition of cholesterol biosynthesis and cell proliferation in a variety of cell types, among them hematopoietic cells. Characterization and differential expression of ORP-3 implicates a possible role in the mediation of oxysterol effects on hematopoiesis.

History

Journal

Blood

Volume

98

Pagination

2279-2281

Location

Washington, D.C.

ISSN

0006-4971

eISSN

1528-0020

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2001, American Society of Hematology

Issue

7

Publisher

American Society of Hematology

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