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Observation of non-linear chromatographic peaks in comprehensive two-dimensional gas chromatography

journal contribution
posted on 2001-05-01, 00:00 authored by R Ong, Robert Shellie, P Marriott
Comprehensive two-dimensional gas chromatography (GC × GC) often incorporates a conventional capillary gas chromatography column in combination with a fast elution column. If this latter column has a thin stationary phase film, it may be supposed that the analyst risks loss of peak resolution due to the onset of non-linear conditions. This will normally be evidenced by overloading - or fronting - peak shapes. GC × GC methods which result from zone compression will be even more likely to exhibit overloading on the second column since the instantaneous peak concentration (CM and CS), will be significantly greater than that of the band which enters the modulation region. Two cases of overloading may be considered - that on the first column, and that on the second column. Each of these cases will lead to different two-dimensional peak shapes when the GC × GC result is converted to a two-dimensional separation space. In each instance, however, resolution of components will be degraded, although the GC × GC experiment will still not be as badly affected as a single dimensional GC analysis. When overloading conditions are obtained, the GC × GC peak pulsing phenomenon will result in the extremities of the chromatographic band showing linear conditions even though the centre of the band is non-linear.

History

Journal

Journal of separation science

Volume

24

Issue

5

Pagination

367 - 377

Publisher

John Wiley & Sons

Location

Chichester, Eng.

ISSN

1615-9306

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2001, WILEY‐VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany

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