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On-line high-performance liquid chromatography/mass spectrometric investigation of amyloid-beta peptide variants found in Alzheimer's disease

journal contribution
posted on 1999-12-15, 00:00 authored by A J Thompson, T K Lim, Colin BarrowColin Barrow
Abeta peptides are the major components of amyloid deposits in Alzheimer's disease. The presence of N-terminally truncated Abeta variants in amyloid may be a critical factor in Alzheimer's disease pathogenesis. These Abeta variants are less soluble and more amyloidogenic than full-length Abeta, making their separation, purification and identification difficult. High-performance liquid chromatography (HPLC) at elevated temperatures, coupled to electrospray ionization (ES) mass spectrometry (MS), enables rapid separation and identification of N-terminally truncated Abeta variants. This methodology provides a potential tool for exploring the importance of these Abeta variants in both the pathogenesis and diagnosis of Alzheimer's disease.

History

Journal

Rapid communications in mass spectrometry

Volume

13

Issue

23

Pagination

2348 - 2351

Publisher

Wiley

Location

Chichester, Eng.

ISSN

0951-4198

eISSN

1097-0231

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1999, Wiley