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Optimal method selection for biocompatible extraction of rosmarinic acid from mycorrhizal hairy roots of Ocimum basilicum

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Version 2 2024-06-03, 15:06
Version 1 2019-05-17, 13:51
journal contribution
posted on 2024-06-03, 15:06 authored by S Srivastava, David CahillDavid Cahill, A Adholeya
© 2019 Mycorrhizal hairy roots of Ocimum basilicum produce high amount of rosmarinic acid and are also valuable resource of quality mycorrhizal spores. To utilize their potential as continuous resource of biological and biochemical products, an efficient separation method is required. Solvent based extraction methods have a negative impact on mycorrhizal spore viability and vitality. Accordingly, we developed a biocompatible extraction method where spore and root viability is maintained with efficient extraction of rosmarinic acid. We screened temperature- and sonication-assisted techniques in ethanol, methanol, dimethyl sulfoxide, ionic liquid and surfactants. An inverse relationship was found between an increase in temperature and mycorrhizal and root viability. Optimum temperature for extraction was 30 °C. Most suitable solvents were 10% methanol; 0.25 M ionic liquid and dimethyl sulfoxide. Ethanol, nonane, dodecane, Triton X-100 and Tween-20 were not found suitable. Thus, our study sets a platform for optimization studies with mycorrhizal roots of other medicinal plants.

History

Journal

Biotechnology reports

Volume

23

Article number

e00325

Location

Amsterdam, The Netherlands

eISSN

2215-017X

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2019, Elsevier B.V.

Publisher

Elsevier

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