File(s) under permanent embargo

Optimization of fatty acid determination in selected fish and microalgal oils

journal contribution
posted on 2009-08-01, 00:00 authored by R Armenta, S Scott, A Burja, H Radianingtyas, Colin BarrowColin Barrow
The use of ten fatty acid methyl ester reference standards coupled with a detailed quantification method was shown to significantly optimize the fatty acid determination of selected fish and microalgal oils when compared to methods that use only one reference standard (C19:0 or C23:0) as a relative response factor. When using the mixture of ten reference standards after transesterifying oils with NaOH/BF3, determination of total fatty acids, eicosapentaenoic acid and docosahexaenoic acid improved by an average of 7.3, 11.5 and 8.4%, respectively. Furthermore, improvements of 13.9, 18.9 and 6.8% of total fatty acids, EPA and DHA, respectively, were obtained when using the mixture of reference standards for fatty acid determination after directly extracting and transesterifying oil contained in microalgal cells with a mixture of methanol, HCl and chloroform. Fatty acid methyl ester standards dissolved in isooctane showed <5% variability throughout 130 days of stability testing when stored at −20 °C. The optimized method can be used for improving the quantification of fatty acids in both oils (fish and microalgal oils) and dry microalgal cells.

History

Journal

Chomatographia

Volume

70

Issue

3-4

Pagination

629 - 636

Publisher

Vieweg Verlag

Location

Berlin, Germany

ISSN

0009-5893

eISSN

1612-1112

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2009, Springer