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Optimization of fatty acid determination in selected fish and microalgal oils
journal contribution
posted on 2009-08-01, 00:00 authored by R Armenta, S Scott, A Burja, H Radianingtyas, Colin BarrowColin BarrowThe use of ten fatty acid methyl ester reference standards coupled with a detailed quantification method was shown to significantly optimize the fatty acid determination of selected fish and microalgal oils when compared to methods that use only one reference standard (C19:0 or C23:0) as a relative response factor. When using the mixture of ten reference standards after transesterifying oils with NaOH/BF3, determination of total fatty acids, eicosapentaenoic acid and docosahexaenoic acid improved by an average of 7.3, 11.5 and 8.4%, respectively. Furthermore, improvements of 13.9, 18.9 and 6.8% of total fatty acids, EPA and DHA, respectively, were obtained when using the mixture of reference standards for fatty acid determination after directly extracting and transesterifying oil contained in microalgal cells with a mixture of methanol, HCl and chloroform. Fatty acid methyl ester standards dissolved in isooctane showed <5% variability throughout 130 days of stability testing when stored at −20 °C. The optimized method can be used for improving the quantification of fatty acids in both oils (fish and microalgal oils) and dry microalgal cells.
History
Journal
ChomatographiaVolume
70Issue
3-4Pagination
629 - 636Publisher
Vieweg VerlagLocation
Berlin, GermanyPublisher DOI
ISSN
0009-5893eISSN
1612-1112Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2009, SpringerUsage metrics
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