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Optimization of process parameters for the production of naringinase by Aspergillus niger MTCC 1344

journal contribution
posted on 2005-01-01, 00:00 authored by Munish Puri, A Banerjee, U Banerjee
Aspergillus niger MTCC 1344 was used to produce extracellular naringinase in a complex (molasses, yeast extract and salts) medium. An initial medium pH 4.5 and cultivation temperature 30 °C were optimal for enzyme production. Among various carbon and organic nitrogen sources used, molasses and peptone were the most effective for enzyme yield. The rate of enzyme production was enhanced when metal ions were added to the medium. Fermentation conditions are described which produced a higher rate of enzyme synthesis. An increase in initial sugar concentration from 6 to 10 g l−1 in the fermentation medium produced decreased naringinase synthesis while cell mass growth increased with the increase of sugar concentration. At a higher sugar level (10 g l−1) the production of cell mass decreased.

History

Journal

Process biochemistry

Volume

40

Issue

1

Pagination

195 - 201

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

1359-5113

eISSN

1873-3298

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2004, Elsevier Ltd