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Organisation of enkephalin inputs and outputs of the central nucleus of the amygdala in mice
journal contribution
posted on 2023-02-10, 05:09 authored by A Viden, SS Ch'ng, LC Walker, A Shesham, SM Hamilton, Craig SmithCraig Smith, AJ LawrenceThe central nucleus of the amygdala (CeA) is a key hub integrating sensory inputs and modulating behavioural outputs. The CeA is a complex structure with discrete subdivisions, high peptidergic heterogeneity and broad CNS afferent and efferent projections. While several neuropeptide systems within the CeA have been examined in detail, less is known about CeA preproenkephalin (ppENK) cells. Here, we used a recently developed transgenic Penk-Cre mouse line to advance our understanding of the efferent and afferent connectivity of ppENK in the CeA. First, to determine the fidelity of Cre expression in Penk-Cre transgenic mice, we conducted RNAscope in the CeA of Penk-Cre mice. Our analysis revealed that 96.6 % of CeA Cre+ neurons co-expressed pENK mRNA, and 99.7 % of CeA pENK+ neurons co-expressed Cre mRNA, indicating faithful recapitulation of Cre expression in CeA ppENK-expressing cells, supporting the fidelity of the Penk-Cre reporter mouse. Anterograde tracing of CeAPenk cells showed strong efferent projections to the extended amygdala, midbrain and hindbrain PBN and NTS. Retrograde tracing of Penk afferents to the CeA were more restricted, with primary innervation originating within the amygdala complex and bed nucleus of the stria terminalis, and minor innervation from the parabrachial nucleus and nucleus of the solitary tract. Together, our data provide a comprehensive map of ENKergic efferent and afferent connectivity of the CeA in Penk-Cre mice. Further, we highlight both the utility and limitations of the Penk-Cre mice to study the function of CeA, PBN and NTS ppENK cells.
History
Journal
Journal of Chemical NeuroanatomyVolume
125Article number
ARTN 102167Location
NetherlandsPublisher DOI
ISSN
0891-0618eISSN
1873-6300Language
EnglishPublication classification
C1 Refereed article in a scholarly journalPublisher
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Keywords
Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyNeurosciencesNeurosciences & NeurologyEnkephalinCentral amygdalaEfferentAfferentConnectivityPenk-CreDOPAMINE NEURONSEXPRESSING NEURONSGABA NEURONSRATCOMPLEXFEARCOEXPRESSIONPROJECTIONSEFFERENTSCIRCUITSMiceAnimalsCentral Amygdaloid NucleusEnkephalinsNeuronsRNA, MessengerGenetics1.1 Normal biological development and functioning1 Underpinning researchNeurosciences not elsewhere classified
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