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Overcoming solvent mismatch limitations in 2D-HPLC with temperature programming of isocratic mobile phases

Version 2 2024-06-04, 05:43
Version 1 2016-03-30, 15:38
journal contribution
posted on 2024-06-04, 05:43 authored by Brendan HollandBrendan Holland, Xavier ConlanXavier Conlan, Paul FrancisPaul Francis, Neil BarnettNeil Barnett, PG Stevenson
This work describes a method for two-dimensional high performance liquid chromatography (2D-HPLC) that uses an isocratic mobile phase with a temperature gradient in the first dimension. Temperature programming was used to manipulate solvent elution strength in place of a mobile phase concentration gradient. This ensured that all eluent fractions transferred into the second dimension were of an identical solvent composition, i.e. the second dimension injection solvent did not increase during the course of the analysis. When applied to a complex natural product extract of coffee, the separation was completed in 35 min and had an orthogonality of 35% (calculated using the bins method) and a spreading angle of 52° as determined via a geometric approach to factor analysis. This approach, incorporating a temperature gradient in the first dimension, compared favourably to previously reported 2D-HPLC separations of coffee, with similar or shorter analysis times.

History

Journal

Analytical methods

Volume

8

Pagination

1293-1298

Location

Cambridge, Eng.

ISSN

1759-9660

eISSN

1759-9679

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2016, Royal Society of Chemistry

Issue

6

Publisher

Royal Society of Chemistry