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Oxidative catabolism of (+)-pinoresinol is initiated by an unusual flavocytochrome encoded by translationally coupled genes within a cluster of (+)-pinoresinol-coinduced genes in pseudomonas sp. strain SG-MS2

Version 2 2024-06-06, 09:49
Version 1 2020-06-15, 12:08
journal contribution
posted on 2024-06-06, 09:49 authored by M Shettigar, S Balotra, A Kasprzak, SL Pearce, MJ Lacey, MC Taylor, JW Liu, David CahillDavid Cahill, JG Oakeshott, G Pandey
(+)-Pinoresinol is an important plant defense compound, a major food lignan for humans and some other animals, and the model compound used to study degradation of the β-β′ linkages in lignin. We report a gene cluster, in one strain each of Pseudomonas and Burkholderia , that is involved in the oxidative catabolism of (+)-pinoresinol. The flavoprotein component of the α-hydroxylase which heads the pathway belongs to the 4-phenol oxidizing (4PO) subgroup of the vanillyl alcohol oxidase/ p -cresol methyl hydroxylase (VAO/PCMH) enzyme family but constitutes a novel combination of cofactor and electron acceptor properties for the family. It is translationally coupled with a cytochrome gene whose product is also required for activity. The work casts new light on the biology of (+)-pinoresinol and its transformation to other bioactive molecules. Potential applications of the findings include new options for deconstructing lignin into useful chemicals and the generation of new phytoestrogenic enterolactones from lignans.

History

Journal

Applied and Environmental Microbiology

Volume

86

Article number

ARTN e00375-20

Pagination

1 - 17

Location

United States

ISSN

0099-2240

eISSN

1098-5336

Language

English

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Issue

10

Publisher

AMER SOC MICROBIOLOGY