yang-parametersimportant-2001.pdf (100.87 kB)
Parameters important in fabricating enzyme electrodes using self-assembled monolayers of alkanethiols
journal contribution
posted on 2001-01-01, 00:00 authored by J Gooding, P Erokhin, D Losic, Wenrong YangWenrong Yang, V Policarpio, J Liu, F Ho, M Situmorang, D Hibbert, J ShapterThe fabrication of enzyme electrodes using self-assembled monolayers (SAMs) has attracted considerable interest because of the spatial control over the enzyme immobilization. A model system of glucose oxidase covalently bound to a gold electrode modified with a SAM of 3-mercaptopropionic acid was investigated with regard to the effect of fabrication variables such as the surface topography of the underlying gold electrode, the conditions during covalent attachment of the enzyme and the buffer used. The resultant monolayer enzyme electrodes have excellent sensitivity and dynamic range which can easily be adjusted by controlling the amount of enzyme immobilized. The major drawback of such electrodes is the response which is limited by the kinetics of the enzyme rather than mass transport of substrates. Approaches to bringing such enzyme electrodes into the mass transport limiting regime by exploiting direct electron transfer between the enzyme and the electrode are outlined.
History
Journal
Analytical sciencesVolume
17Issue
1Pagination
3 - 9Publisher
Japan Society for Analytical ChemistryLocation
Tokyo, JapanISSN
1348-2246eISSN
0910-6340Language
engNotes
Reproduced with the kind permission of the copyright owner.Publication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2001, Japan Society for Analytical ChemistryUsage metrics
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