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Performance of columns packed with the new shell particles, Kinetex-C18

journal contribution
posted on 2010-03-05, 00:00 authored by F Gritti, I Leonardis, D Shock, Paul Stevenson, A Shalliker, G Guiochon
The performance of the new Kinetex-C18 column was investigated. Packed with a new brand of porous shell particles, this column has an outstanding efficiency. Once corrected for the contribution of the instrument extra column volume, the minimum values of the reduced plate heights for a number of low molecular weight compounds (e.g., anthracene and naphtho[2,3-a]pyrene) were between 1.0 and 1.3, breaking the legendary record set 3 years ago by Halo-C18 packed columns. The liquid-solid mass transfer of proteins (e.g., insulin and lyzozyme) is exceptionally fast on Kinetex-C18 much faster than on the Halo-C18 column. The different contributions of dispersion and mass transfer resistances to the column efficiency were determined and discussed. The possible reasons for this extremely high column efficiency are discussed.

History

Journal

Journal of chromatography A

Volume

1217

Issue

10

Pagination

1589 - 1603

Publisher

Elsevier BV

Location

Amsterdam, The Netherlands

ISSN

0021-9673

eISSN

1873-3778

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2010, Elsevier B.V.