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Peroxide reduction by a metal-dependent catalase in Nostoc punctiforme (cyanobacteria)
journal contribution
posted on 2017-05-01, 00:00 authored by Lee Hudek, Angel TorrieroAngel Torriero, Agnes MichalczykAgnes Michalczyk, B A Neilan, Leigh AcklandLeigh Ackland, Lambert BrauLambert BrauThis study investigated the role of a novel metal-dependent catalase (Npun_R4582) that reduces hydrogen peroxide in the cyanobacterium Nostoc punctiforme. Quantitative real-time PCR showed that npun_R4582 relative mRNA levels were upregulated by over 16-fold in cells treated with either 2 µM added Co, 0.5 µM added Cu, 500 µM Mn, 1 µM Ni or 18 µM Zn. For cells treated with 60 µM H2O2, no significant alteration in Npun_R4582 relative mRNA levels was detected, while in cells treated with Co, Cu, Mn, Ni or Zn and 60 µM peroxide, relative mRNA levels were generally above control or peroxide only treated cells. Disruption or overexpression of npun_R4582 altered sensitivity to cells exposed to 60 µM H2O2 and metals for treatments beyond the highest viable concentrations, or in a mixed metal solution for Npun_R4582- cells. Moreover, overexpression of npun_R4582 increased cellular peroxidase activity in comparison with wildtype and Npun_R4582- cells, and reduced peroxide levels by over 50%. The addition of cobalt, manganese, nickel and zinc increased the capacity of Npun_R4582 to reduce the rate or total levels of peroxide produced by cells growing under photooxidative conditions. The work presented confirms the function of NpunR4582 as a catalase and provides insights as to how cells reduce potentially lethal peroxide levels produced by photosynthesis. The findings also show how trace elements play crucial roles as enzymatic cofactors and how the role of Npun_R4582 in hydrogen peroxide breakdown is dependent on the type of metal and the level available to cells.
History
Journal
Applied microbiology and biotechnologyVolume
101Issue
9Pagination
3781 - 3800Publisher
SpringerLocation
Berlin, GermanyPublisher DOI
ISSN
0175-7598eISSN
1432-0614Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2017, Springer-VerlagUsage metrics
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No categories selectedKeywords
reactive oxygen species (ROS)catalaseperoxdidasecyanobacteriatrace elementsScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyPeroxidaseMULTIPLE SEQUENCE ALIGNMENTOXIDATIVE STRESSMEDICAGO-TRUNCATULAELECTRON-TRANSPORTMANGANESE CATALASEESCHERICHIA-COLIZINCHOMEOSTASISRESISTANCEEXPRESSION
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