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Phylogenetic and Expression Analysis of Fos Transcription Factors in Zebrafish
journal contributionposted on 2023-02-10, 03:11 authored by K Kubra, GK Gaddu, Clifford LiongueClifford Liongue, S Heidary, Alister WardAlister Ward, Amardeep DhillonAmardeep Dhillon, Faiza BasheerFaiza Basheer
Members of the FOS protein family regulate gene expression responses to a multitude of extracellular signals and are dysregulated in several pathological states. Whilst mouse genetic models have provided key insights into the tissue-specific functions of these proteins in vivo, little is known about their roles during early vertebrate embryonic development. This study examined the potential of using zebrafish as a model for such studies and, more broadly, for investigating the mechanisms regulating the functions of Fos proteins in vivo. Through phylogenetic and sequence analysis, we identified six zebrafish FOS orthologues, fosaa, fosab, fosb, fosl1a, fosl1b, and fosl2, which show high conservation in key regulatory domains and post-translational modification sites compared to their equivalent human proteins. During embryogenesis, zebrafish fos genes exhibit both overlapping and distinct spatiotemporal patterns of expression in specific cell types and tissues. Most fos genes are also expressed in a variety of adult zebrafish tissues. As in humans, we also found that expression of zebrafish FOS orthologs is induced by oncogenic BRAF-ERK signalling in zebrafish melanomas. These findings suggest that zebrafish represent an alternate model to mice for investigating the regulation and functions of Fos proteins in vertebrate embryonic and adult tissues, and cancer.
JournalInternational Journal of Molecular Sciences
Article numberARTN 10098
Publication classificationC1 Refereed article in a scholarly journal
Science & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemistry & Molecular BiologyChemistry, MultidisciplinaryChemistryFosactivator protein-1transcription factorembryogenesiszebrafishdevelopmentorthologC-FOSCELL-PROLIFERATIONFAMILY-MEMBERSAP-1 SUBUNITSMICE LACKINGACTIVATIONTRANSFORMATIONFRA-1GENEJUNAnimalsEmbryonic DevelopmentGene Expression Regulation, DevelopmentalHumansPhylogenyProto-Oncogene Proteins c-fosTranscription FactorsZebrafishZebrafish ProteinsGenetics1 Underpinning research1.1 Normal biological development and functioningGeneric health relevanceChemical Sciences not elsewhere classifiedGeneticsBiological Sciences not elsewhere classified