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Physicochemical and blood compatibility characterization of polypyrrole surface functionalized with heparin

journal contribution
posted on 2003-11-05, 00:00 authored by Yali Li, K G Neoh, L Cen, E T Kang
A surface modification technique was developed for the covalent immobilization of heparin onto electrically conductive polypyrrole (PPY) film. The PPY film was first graft copolymerized with poly(ethylene glycol) methacrylate (PEGMA) and then activated with cyanuric chloride (CC). Heparin was then immobilized onto the film through the reaction between the chloride groups of CC and the amine and/or hydroxyl groups of heparin. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface-modified film after each stage. The biocompatibility of the surface-modified PPY was evaluated using plasma recalcification time (PRT) and platelet adhesion. After surface modification, the film had improved wettability while retaining significant electrical conductivity. With immobilized heparin, platelet adhesion and platelet activation on PPY film was significantly suppressed, and the PRT was significantly prolonged. Electrical stimulation also plays a positive role in decreasing platelet adhesion and increasing PRT on pristine and surface-modified PPY films.

History

Journal

Biotechnology and bioengineering

Volume

84

Issue

3

Pagination

305 - 313

Publisher

John Wiley & Sons

Location

Chichester, Eng.

ISSN

0006-3592

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2003, Wiley Periodicals, Inc.