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Plastid division in mallomonas (Synurophyceae, Heterokonta)
journal contribution
posted on 2007-05-16, 00:00 authored by K Weatherill, I Lambiris, J Pickett-Heaps, J Deane, Peter BeechPeter BeechLaser scanning confocal microscopy and TEM were used to study the morphology of secondary plastids in algae of the genus Mallomonas (Synurophyceae). At interphase, Mallomonas splendens (G. S. West) Playfair, M. rasilis Dürrschm., M. striata Asmund, and M. adamas K. Harris et W. H. Bradley contained a single H-shaped plastid consisting of two large lobes connected by a narrow isthmus. Labeling of DNA revealed a necklace-like arrangement of plastid nucleoids at the periphery of the M. splendens plastid and a less-patterned array in M. rasilis. The TEM of M. splendens and M. rasilis showed an electron-dense belt surrounding the plastid isthmus in interphase cells; this putative plastid-dividing ring (PD ring) was adpressed to the inner pair of the four plastid membranes, suggesting that it is homologous to the PD ring of green and red plastids. The PD ring did not contain actin (indicated by lack of staining with phalloidin) and displayed filaments or tubules of 5–10 nm in diameter that may be homologous to the tubules described in red algal PD rings. Confocal microscopy of chl autofluorescence from M. splendens showed that the plastid isthmus was severed as mitosis began, giving rise to two single-lobed daughter plastids, which, as mitosis and cell division progressed, separated from one another and then each constricted to form the H-shaped plastids of daughter cells. Similar plastid division cycles were observed in M. rasilis and M. adamas; however, the plastid isthmus of M. striata was retained throughout most of cell division and was eventually severed by the cell cleavage furrow.
History
Journal
Journal of PhycologyVolume
43Issue
3Pagination
535 - 541Publisher
John Wiley and Sons Ltd.Location
Maiden, Mass.Publisher DOI
ISSN
0022-3646eISSN
1529-8817Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2007, Phycological Society of AmericaUsage metrics
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Keywords
chloroplastMallomonasorganelleplastidplastid-dividing ringsecondary endosymbiosisScience & TechnologyLife Sciences & BiomedicinePlant SciencesMarine & Freshwater Biologydividing ringSPLENDENS SYNUROPHYCEAECELL-CYCLECHLOROPLAST NUCLEOIDSMITOCHONDRIALAPPARATUSPROTEINFTSZCHLOROPHYTABACTERIALRINGSPlant Biology
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