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Precision milled flow-cells for chemiluminescence detection
journal contribution
posted on 2009-01-01, 00:00 authored by S Mohr, Jessica Terry, Jacqui AdcockJacqui Adcock, P Fielden, N Goddard, Neil BarnettNeil Barnett, D Wolcott, Paul FrancisPaul FrancisNovel flow-cells with integrated confluence points and reaction channels designed for efficient mixing of fast chemiluminescence systems were constructed by machining opposing sides of a polymer chip and sealing the channels with transparent epoxy-acetate films. A hole drilled through the chip provided the conduit from the confluence point on one side to the centre of the reaction zone on the other side, allowing rapid presentation of the reacting mixture to the photodetector. The effectiveness of each flow-cell was evaluated by comparing the chemiluminescence intensity using flow injection analysis methodology, and examining the distribution of light emanating from the reaction zone (captured by photography in a dark room) when the reactants were continuously merged. Although previously reported chemiluminescence detectors constructed by machining channels into polymers have almost exclusively been prepared using transparent materials, we obtained far greater emission intensities using an opaque white chip with a thin transparent seal, which minimised the loss of light through surfaces not exposed to the photomultiplier tube. Furthermore, this approach enabled the exploration of reactor designs that could not be incorporated in traditional coiled-tubing flow-cells.
History
Journal
AnalystVolume
134Issue
11Pagination
2233 - 2238Publisher
Royal Society of ChemistryLocation
Cambridge, EnglandPublisher DOI
ISSN
0003-2654eISSN
1364-5528Language
engNotes
First published as an Advance Article on the web 10th September 2009Publication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2009, Royal Society of ChemistryUsage metrics
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