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Preliminary results of the application of a silk fibroin scaffold to otology

journal contribution
posted on 01.03.2010, 00:00 authored by B Levin, S Redmond, Rangam RajkhowaRangam Rajkhowa, R Eikelboom, R Marano, M Atlas
The surgical treatment to repair chronic tympanic membrane perforations is myringoplasty. Although multiple autologous grafts, allografts, and synthetic graft materials have been used over the years, no single graft material is superior for repairing all perforation types. Recently, the remarkable properties of silk fibroin protein have been studied, with biomedical and tissue engineering applications in mind, across a number of medical and surgical disciplines. The present study examines the use of silk fibroin for its potential suitability as an alternative graft in myringoplasty surgery by investigating the growth and proliferation of human tympanic membrane keratinocytes on a silk fibroin scaffold in vitro. Light microscopy, immunofluorescent staining, and confocal imaging all reveal promising preliminary results. The biocompatibility, transparency, stability, high tensile strength, and biodegradability of fibroin make this biomaterial an attractive option to study for this utility.

History

Journal

Otolaryngology–head and neck surgery

Volume

142

Issue

3

Season

Supplement 1

Publisher

Mosby, Inc.

Location

Philadelphia, Pa.

ISSN

0194-5998

eISSN

1097-6817

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2010, American Academy of Otolaryngology–Head and Neck Surgery Foundation. All rights reserved.