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Prevailing genotype distribution and characteristics of human respiratory syncytial virus in northeastern China
journal contributionposted on 2023-08-11, 03:22 authored by Yuxuan Zheng, Li Liu, Shaohua Wang, Zhaolong Li, Min Hou, Jingliang LiJingliang Li, Xiao-Fang Yu, Wenyan Zhang, Shucheng Hua
Although human respiratory syncytial virus (RSV) is one of the most common viruses inducing respiratory tract infections in young children and the elderly, the genotype distribution and characteristics of RSV in northeastern China have not been investigated. Here, we identified 25 RSV-A and 8 RSV-B strains from 80 samples of patients with respiratory infections between February 2015 and May 2015. All 25 RSV-A viruses were classified as the ON1 genotype, which rapidly spread and became the dominant genotype in the world since being identified in Ontario (Canada) in December 2010. All eight RSV-B viruses belonged to the BA genotype with a 60-nucleotide duplication, seven of which formed two new genotypes, BA-CCA and BA-CCB. The remaining RSV-B virus clustered with one of the Hangzhou strains belonging to genotype BA11. Construction of a phylogenetic tree and amino acid substitution analysis showed that Changchun ON1 viruses exclusively constituted Lineages 3, 5 and 6, and contained several unique and newly identified amino acid substitutions, including E224G, R244K, L289I, Y297H, and L298P. Selective pressure was also evaluated, and various N and O-glycosylation sites were predicted. This study provides the first genetic analysis of RSV in northeastern China and may facilitate a better understanding of the evolution of this virus locally and globally. J. Med. Virol. 89:222-233, 2017. © 2016 The Authors. Journal of Medical Virology Published by Wiley Periodicals, Inc.
JournalJOURNAL OF MEDICAL VIROLOGY
Publication classificationC1.1 Refereed article in a scholarly journal
Science & TechnologyLife Sciences & BiomedicineVirologyrespiratory syncytial virusgenetic variabilitymutationmutation rateglycoproteinsATTACHMENT GLYCOPROTEIN-GG-PROTEINMOLECULAR EPIDEMIOLOGYGENETIC DIVERSITYON1 GENOTYPESUBGROUP-ACONSECUTIVE SEASONSRAPID SPREADEVOLUTIONSTRAINSmutation/mutation rateAmino Acid SubstitutionChild, PreschoolChinaCluster AnalysisFemaleGenotypeHumansInfantInfant, NewbornMalePhylogenyPrevalenceRNA, ViralRespiratory Syncytial Virus InfectionsRespiratory Syncytial Virus, HumanSelection, GeneticSequence Analysis, DNA3207 Medical Microbiology32 Biomedical and Clinical Sciences31 Biological SciencesInfectious DiseasesGeneticsInfection3107 Microbiology3202 Clinical sciences3207 Medical microbiologyMicrobiologyMedical Microbiology not elsewhere classified