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Primers for novel microsatellite markers in "fire-specialist" lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations

Version 2 2024-06-04, 05:51
Version 1 2015-09-30, 17:05
journal contribution
posted on 2024-06-04, 05:51 authored by AL Smith, MG Gardner, CM Bull, Don DriscollDon Driscoll
We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2-24 alleles and expected heterozygosities of 0.043-0.927. These markers will facilitate studies of post-fire dispersal and recolonisation. © Springer Science+Business Media B.V. 2010.

History

Journal

Conservation genetics resources

Volume

3

Pagination

345-350

Location

Berlin, Germany

ISSN

1877-7252

eISSN

1877-7260

Language

eng

Publication classification

C Journal article, C1.1 Refereed article in a scholarly journal

Copyright notice

2011, Springer

Issue

2

Publisher

Springer