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Primers for novel microsatellite markers in "fire-specialist" lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations
journal contributionposted on 2011-04-01, 00:00 authored by A L Smith, M G Gardner, C M Bull, Don DriscollDon Driscoll
We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2-24 alleles and expected heterozygosities of 0.043-0.927. These markers will facilitate studies of post-fire dispersal and recolonisation. © Springer Science+Business Media B.V. 2010.