shigdar-profilingcancercells-2022.pdf (1.54 MB)
Profiling cancer cells by cell-selex: Use of aptamers for discovery of actionable biomarkers and therapeutic applications thereof
journal contribution
posted on 2022-01-01, 00:00 authored by Sarah ShigdarSarah Shigdar, L Agnello, M Fedele, S Camorani, L CerchiaThe identification of tumor cell-specific surface markers is a key step towards personalized cancer medicine, allowing early assessment and accurate diagnosis, and development of efficacious targeted therapies. Despite significant efforts, currently the spectrum of cell membrane targets associated with approved treatments is still limited, causing an inability to treat a large number of cancers. What mainly limits the number of ideal clinical biomarkers is the high complexity and heterogeneity of several human cancers and still-limited methods for molecular profiling of specific cancer types. Thanks to the simplicity, versatility and effectiveness of its application, cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) technology is a valid complement to the present strategies for biomarkers’ discovery. We and other researchers worldwide are attempting to apply cell-SELEX to the generation of oligonucleotide aptamers as tools for both identifying new cancer biomarkers and targeting them by innovative therapeutic strategies. In this review, we discuss the potential of cell-SELEX for increasing the currently limited repertoire of actionable cancer cell-surface biomarkers and focus on the use of the selected aptamers as components of innovative conjugates and nano-formulations for cancer therapy.
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Journal
PharmaceuticsVolume
14Issue
1Article number
28Pagination
1 - 20Publisher
MDPI AGLocation
Basel, SwitzerlandPublisher DOI
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1999-4923eISSN
1999-4923Language
engPublication classification
C1 Refereed article in a scholarly journalUsage metrics
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aptameraptamer guided-nanomedicinesaptamer-antibody conjugatesbiomarker discoverycancer cell phenotypecell-profilingcell-SELEXtargeted deliverytargeted therapyScience & TechnologyLife Sciences & BiomedicinePharmacology & PharmacyCONTROLLED-RELEASE DELIVERYIN-VITRO SELECTIONDNA APTAMERSTRANSFERRIN RECEPTORMOLECULAR PROBESRNA APTAMERSSYSTEMATIC EVOLUTIONTUMOR-GROWTHSTEM-CELLS
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