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Profiling of secondary metabolites in blue lupin inoculated with Phytophthora cinnamomi following phosphite treatment

journal contribution
posted on 2013-01-01, 00:00 authored by Tiffany Gunning, Xavier ConlanXavier Conlan, R Parker, Gail Dyson, M Adams, Neil BarnettNeil Barnett, David CahillDavid Cahill
In order to discover phytochemicals that are potentially bioactive against Phytophthora cinnamomi, (a soil-borne plant pathogen) a metabolite profiling protocol for investigation of metabolic changes in Lupinus angustifolius L. plant roots in response to pathogen challenge has been established. Analysis of the metabolic profiles from healthy and P. cinnamomi-inoculated root tissue with high resolution mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that although susceptible, L. angustifolius upregulated a defence associated genistein and 2′-hydroxygenistein-based isoflavonoid and a soyasapogenol saponin at 12h post inoculation which increased in concentration at 72h post inoculation. In contrast to the typical susceptible interaction, the application of a phosphorous-based treatment to L. angustifolius foliage 48h before P. cinnamomi challenge negated the ability of the pathogen to colonise the root tissue and cause disease. Importantly, although the root profiles of water-treated and phosphite-treated plants post pathogen inoculation contained the same secondary metabolites, concentration variations were observed. Accumulation of secondary metabolites within the P. cinnamomi-inoculated plants confirms that pathogen ingress of the root interstitially occurs in phosphite-treated plants, confirming a direct mode of action against the pathogen upon breaching the root cells.

History

Journal

Functional plant biology

Volume

40

Issue

11

Pagination

1089 - 1097

Publisher

CSIRO Publishing

Location

Melbourne, Vic.

ISSN

1445-4408

eISSN

1445-4416

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2013, CSIRO Publishing