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Properties of an in vitro selected Pb2+ cleavage motif
journal contribution
posted on 1994-08-01, 00:00 authored by T Pan, Bernhard DichtlBernhard Dichtl, O UhlenbeckThe addition of Pb 2+ to a small RNA molecule consisting of an asymmetric internal loop of six nucleotides results in site-specific cleavage followed by hydrolysis of the 2′,3′-cyclic phosphate intermediate [Pan, T., & Uhlenbeck, O. C. (1992) Nature 358, 560-563]. Here we show that the reaction is highly specific for Pb 2+ and the cleavage rate increases exponentially with pH from 5.5 to 7.0, both in the presence and in the absence of Mg 2+. This suggests that the reaction mechanism involves Pb 2+ hydroxide acting as a base. Several sequence variants of the RNA are found to be equally active in both steps of the reaction, suggesting that they fold into a similar structure.
History
Journal
BiochemistryVolume
33Issue
32Pagination
9561 - 9565Publisher
American Chemical SocietyLocation
Washington, D. C.Publisher DOI
ISSN
0006-2960eISSN
1520-4995Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1994, American Chemical SocietyUsage metrics
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