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Pulsed flow chemistry : a new approach to solution handling for flow analysis coupled with chemiluminescence detection

Version 2 2024-06-03, 22:42
Version 1 2015-08-21, 11:41
journal contribution
posted on 2024-06-03, 22:42 authored by S Lewis, Paul FrancisPaul Francis, Kieran LimKieran Lim, GE Jenkins, XD Wang
Novel instrumentation for flow analysis, coupled with chemiluminescence detection, and based on pulsed flow chemistry is described. The pulsed flow chemistry manifold allows for rapid and efficient mixing of chemiluminescent reagent with sample immediately in front of the detector, thus giving high precision (less than 1 % RSD) and detectability, particularly for rapid, short-lived emissions. The instrument is also characterised by a compact size, high sampling frequency (120 to 300 samples per hour) and low reagent consumption (less than 500 ml per cycle). Other flow analysis modes (eg. flow injection, continuous flow and stopped flow) are available by simply altering software settings. The suitability of the system was shown using chemiluminescence reactions of analytical significance, namely the oxidation of luminol with hexacyanoferrate(III) in the presence of 4-hydroxy-3-methoxybenzeneacetic acid (homovanillic acid), and the reduction of acidic potassium permanganate by morphine in the presence of polyphosphates. The latter reaction was used to determine morphine in aqueous process samples and the results obtained with the pulsed flow instrument displayed good agreement (mean difference of 1.8%) with a previously validated flow injection analysis method.

History

Journal

Analyst

Volume

125

Pagination

1869-1874

Location

London, Eng.

ISSN

0003-2654

Language

eng

Publication classification

C Journal article, C1.1 Refereed article in a scholarly journal

Copyright notice

2000, The Royal Society of Chemistry

Issue

10

Publisher

Royal Society of Chemistry