alexandersen-quantitiesofinfectious-2002.pdf (582.77 kB)
Quantities of infectious virus and viral RNA recovered from sheep and cattle experimentally infected with foot-and-mouth disease virus O UK 2001
journal contribution
posted on 2002-08-01, 00:00 authored by Soren AlexandersenSoren Alexandersen, Z Zhang, S M Reid, G H Hutchings, A I DonaldsonThe profiles of virus production and excretion have been established for sheep experimentally infected with the UK 2001 strain of foot-and-mouth disease (FMD) virus by inoculation and by direct and intensive contact. Virus replicated rapidly in the inoculated sheep, from which a peak infectivity of airborne virus of 10(4.3) TCID(50) per sheep per 24 h was recovered. Around 24 h later, contact-infected sheep excreted airborne virus maximally. Similar amounts of airborne virus were recovered from cattle. The excretion of virus by the sheep under these conditions fell into three phases. First, a highly infectious period of around 7-8 days. Second, a period of 1-3 days soon afterwards when trace amounts of viral RNA were recovered in nasal and rectal swabs. Third, at 4 weeks after exposure, the demonstration, by tests on oesophageal-pharyngeal samples, that 50% of the sheep were carriers. The implications of the results and the variable role that sheep may play in the epidemiology of FMD are discussed.
History
Journal
Journal of general virologyVolume
83Issue
8Pagination
1915 - 1923Publisher
Microbiology SocietyLocation
London, Eng.Publisher DOI
Link to full text
ISSN
0022-1317Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2002, SGMUsage metrics
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AerosolsAir MicrobiologyAnimalsAntibodies, ViralCattleCattle DiseasesFoot-and-Mouth DiseaseFoot-and-Mouth Disease VirusNoseRNA, ViralRectumSheepSheep DiseasesSpecimen HandlingVirus SheddingScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyVirologyLINKED IMMUNOSORBENT-ASSAYAIRBORNE SPREADPCR ASSAYRT-PCRDIAGNOSISELISAANTIBODIESTISSUESPIGS
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