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Quorum-sensing signal production by Agrobacterium vitis strains and their tumor-inducing and tartrate-catabolic plasmids.

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posted on 2009-07-01, 00:00 authored by N Lowe, Han Ming Gan, V Chakravartty, R Scott, E Szegedi, T J Burr, M A Savka
Agrobacterium vitis strains, their tumor-inducing (pTi) and tartrate utilization (pTr) plasmid transconjugants and grapevine tumors were analyzed for the presence of N-acyl-homoserine lactones (AHLs). All wild-type A. vitis strains produced long-chain signals. PCR analysis of the A. vitis long-chain AHL synthase gene, avsI, showed the predicted amplicon. Agrobacterium tumefaciens UBAPF2 harboring various A. vitis pTi plasmids produced N-(3-oxo-octanoyl)-l-homoserine lactone encoded also by pTis of A. tumefaciens. UBAPF2 transconjugants carrying pTrs except for pTrTm4 and pTrAB3, also produced an AHL. UBAPF2 transconjugants carrying pTrAT6, pTrAB4 and pTrRr4 or pTiNi1 produced two additional AHLs not observed in the corresponding wild-type strains. We also provide evidence for in situ production of AHLs in grapevine crown gall tumors of greenhouse and field origin.

History

Journal

Fems Microbiology Letters

Volume

296

Issue

1

Pagination

102 - 109

Publisher

Oxford University Press (OUP)

Location

England

ISSN

0378-1097

eISSN

1574-6968

Language

eng

Publication classification

C Journal article; C1.1 Refereed article in a scholarly journal