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Rapid Point-of-Care Electrochemical Sensor for the Detection of Cancer Tn Antigen Carbohydrate in Whole Unprocessed Blood
journal contributionposted on 2023-02-20, 03:29 authored by SM Silva, DP Langley, LR Cossins, AN Samudra, AF Quigley, RMI Kapsa, RW Tothill, Wren Greene, SE Moulton
Improving outcomes for cancer patients during treatment and monitoring for cancer recurrence requires personalized care which can only be achieved through regular surveillance for biomarkers. Unfortunately, routine detection for blood-based biomarkers is cost-prohibitive using currently specialized laboratories. Using a rapid self-assembly sensing interface amenable to methods of mass production, we demonstrate the ability to detect and quantify a small carbohydrate-based cancer biomarker, Tn antigen (αGalNAc-Ser/Thr) in a small volume of blood, using a test format strip reminiscent of a blood glucose test. The detection of Tn antigen at picomolar levels is achieved through a new transduction mechanism based on the impact of Tn antigen interactions on the molecular dynamic motion of a lectin cross-linked lubricin antifouling brush. In tests performed on retrospective blood plasma samples from patients presenting three different tumor types, differentiation between healthy and diseased patients was achieved, highlighting the clinical potential for cancer monitoring.
Publication classificationC1 Refereed article in a scholarly journal
PublisherAMER CHEMICAL SOC
Science & TechnologyPhysical SciencesChemistry, MultidisciplinaryChemistry, AnalyticalNanoscience & NanotechnologyChemistryScience & Technology - Other TopicsTn antigenlubricincancer biomarkerslectin-based diagnosticspoint-of-care electrochemical sensorantifouling coatingsabnormal O-glycansliquid biopsiesSIALYL-TNLUBRICINREAGENTLESSAGGLUTININADSORPTIONELECTRODESSURFACESHumansPoint-of-Care SystemsRetrospective StudiesNeoplasmsCarbohydratesCancerPreventionClinical Research4.1 Discovery and preclinical testing of markers and technologies4 Detection, screening and diagnosisBiomedical Engineering not elsewhere classifiedAnalytical Chemistry not elsewhere classifiedNanotechnology not elsewhere classified