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Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA
Version 2 2024-06-05, 07:52Version 2 2024-06-05, 07:52
Version 1 2020-06-15, 11:43Version 1 2020-06-15, 11:43
journal contribution
posted on 2024-06-05, 07:52 authored by L Ma, Azadeh Nilghaz, JR Choi, X Liu, X Lu© 2017 Elsevier Ltd In this study, a paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) was developed as a screening system for rapid detection of clenbuterol, which is illegally used as a growth promoter for food-producing animals. The microfluidic paper-based analytical device (μPAD) was combined with ELISA and the intrinsic properties of paper allowed the entrapment of antibody through cellulosic fibres, validating to be an alternative to 96-well ELISA microplate for food safety monitoring. Detection of clenbuterol in milk was achieved by measuring the intensity of colour change that was proportional to the analyte concentration with a detection limit of 0.2 ppb. The μPAD effectively reduces the cost, volume of reagents, and time required to run ELISA for food sample testing.
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Journal
Food ChemistryVolume
246Pagination
437-441Location
Amsterdam, The NetherlandsPublisher DOI
ISSN
0308-8146eISSN
1873-7072Language
engPublication classification
C1 Refereed article in a scholarly journalPublisher
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