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Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA

Version 2 2024-06-05, 07:52
Version 1 2020-06-15, 11:43
journal contribution
posted on 2024-06-05, 07:52 authored by L Ma, Azadeh Nilghaz, JR Choi, X Liu, X Lu
© 2017 Elsevier Ltd In this study, a paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) was developed as a screening system for rapid detection of clenbuterol, which is illegally used as a growth promoter for food-producing animals. The microfluidic paper-based analytical device (μPAD) was combined with ELISA and the intrinsic properties of paper allowed the entrapment of antibody through cellulosic fibres, validating to be an alternative to 96-well ELISA microplate for food safety monitoring. Detection of clenbuterol in milk was achieved by measuring the intensity of colour change that was proportional to the analyte concentration with a detection limit of 0.2 ppb. The μPAD effectively reduces the cost, volume of reagents, and time required to run ELISA for food sample testing.

History

Journal

Food Chemistry

Volume

246

Pagination

437-441

Location

Amsterdam, The Netherlands

ISSN

0308-8146

eISSN

1873-7072

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Publisher

Elsevier