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Rapid determination of carbohydrates in heroin drug seizures using capillary electrophoresis with short-end injection
journal contribution
posted on 2005-09-01, 00:00 authored by N Anastos, S Lewis, Neil BarnettNeil Barnett, J Pearson, P KirkbrideA simple and rapid method for the analysis of carbohydrates in heroin samples by capillary electrophoresis utilizing a borate complexation method is described. Separations were performed using an uncoated fused silica capillary, 50 cm × 50 mm I.D. × 360 mm O.D. with an effective separation length of 9 cm. The system was run at 60°C with an applied voltage of -8 kilovolts. Injection of each sample was for 1 sec at -50 mbar. UV detection was employed with the wavelength set at 195 nm. The background electrolyte consisted of 65 mM borate, pH 12.0. Samples and standards were prepared in the run buffer containing 2 mg/mL of mannose as an internal standard. Under these conditions a test mixture containing glucose, sucrose, lactose, mannitol and mannose as an internal standard was resolved within 5 min. The method was used to determine the concentration of carbohydrates in heroin seizure samples and synthetic heroin samples. The results were in good agreement with the reported values.
History
Journal
Journal of forensic sciencesVolume
50Issue
5Pagination
1039 - 1043Publisher
American Society for Testing and MaterialsLocation
West Conshohocken, Pa.ISSN
0022-1198eISSN
1556-4029Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2005, American Society for Testing and MaterialsUsage metrics
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No categories selectedKeywords
capillary electrophoresisforensic scienceheroin seizuresshort-end injectionScience & TechnologyLife Sciences & BiomedicineMedicine, LegalLegal MedicinecarbohydratesborateINDIRECT UV DETECTIONPERFORMANCE LIQUID-CHROMATOGRAPHYLASER-DESORPTION\/IONIZATION-TIMEFLIGHT-MASS-SPECTROMETRYZONE-ELECTROPHORESISCHIRAL SEPARATIONAMINO-ACIDSELECTROCHEMICAL DETECTIONCLANDESTINE TABLETSFORENSIC ANALYSIS
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