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Rapid determination of papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection
journal contribution
posted on 2007-07-30, 00:00 authored by Jason Wesley Costin, S Lewis, Stuart Purcell, L Waddell, Paul FrancisPaul Francis, Neil BarnettNeil BarnettWe have combined high-performance liquid chromatography (HPLC) separations using a monolithic column with acidic potassium permanganate and tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection in a rapid and highly sensitive method to monitor the process of extracting opiate alkaloids from Papaver somniferum. Due to the high flow rates allowed with the monolithic column and the inherent selectivity of the chemiluminescence reactions, the four predominant alkaloids – morphine, codeine, oripavine and thebaine – were determined in less than 2 min. The results obtained with numerous process samples compared favourable with those of the standard HPLC methodology. Limits of detection were 1 × 10−10 M, 5 × 10−10 M, 5 × 10−10 M and 1 × 10−9 M, for morphine, codeine, oripavine and thebaine, respectively.
History
Journal
Analytica chimica actaVolume
597Issue
1Pagination
19 - 23Publisher
Elsevier BVLocation
Amsterdam, NetherlandsPublisher DOI
ISSN
0003-2670eISSN
1873-4324Language
engNotes
Available online 27 June 2007.Publication classification
C1 Refereed article in a scholarly journalCopyright notice
2007, Elsevier B.V.Usage metrics
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monolithic columnalkaloidschemiluminescenceScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistryACIDIC POTASSIUM-PERMANGANATEFLOW-INJECTION ANALYSISTRIS(2,2'-BIPYRIDYL)RUTHENIUM(II) CHEMILUMINESCENCECAPILLARY-ELECTROPHORESISELECTROCHEMICAL DETECTIONMORPHINESILICAHPLCFLUORESCENCESEPARATION
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