callahan-rapidinduction-2018.pdf (1.13 MB)
Rapid induction of vitamin B12 deficiency in Caenorhabditis elegans cultured in axenic medium
journal contribution
posted on 2018-09-01, 00:00 authored by S M Tegegne, M Jois, M R Flavel, Damien CallahanDamien Callahan, D BenheimAnimal models of B12 deficiency have proven to be difficult due to storage of substantial amounts in the liver and the length of time required to maintain animals on a B12 deficient diet to induce deficiency. Caenorhabditis elegans (C. elegans), due to its short lifespan, has recently emerged as an alternate model to investigate vitamin B12 (B12) deficiency. However, when C. elegans are maintained on bacterial diet, five generations of B12 deficient diet is required before the worms show signs of deficiency. Here we show that C. elegans grown in chemically defined axenic medium without added B12 exhibit signs of deficiency within one generation. Worms grown in deficient media had lower cobalt concentration, retarded growth, reduced fertility, increased motility, reduced quiescence and a shortened lifespan. In conclusion, C. elegans cultured in a defined axenic medium is a suitable and rapid model for studies on B12 deficiency.
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Journal
Journal of nutrition and intermediary metabolismVolume
13Pagination
20 - 25Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
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2352-3859Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2018, The AuthorsUsage metrics
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