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Rapid induction of vitamin B12 deficiency in Caenorhabditis elegans cultured in axenic medium

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Version 2 2024-06-03, 20:11
Version 1 2018-09-10, 14:09
journal contribution
posted on 2024-06-03, 20:11 authored by SM Tegegne, M Jois, MR Flavel, Damien CallahanDamien Callahan, D Benheim
Animal models of B12 deficiency have proven to be difficult due to storage of substantial amounts in the liver and the length of time required to maintain animals on a B12 deficient diet to induce deficiency. Caenorhabditis elegans (C. elegans), due to its short lifespan, has recently emerged as an alternate model to investigate vitamin B12 (B12) deficiency. However, when C. elegans are maintained on bacterial diet, five generations of B12 deficient diet is required before the worms show signs of deficiency. Here we show that C. elegans grown in chemically defined axenic medium without added B12 exhibit signs of deficiency within one generation. Worms grown in deficient media had lower cobalt concentration, retarded growth, reduced fertility, increased motility, reduced quiescence and a shortened lifespan. In conclusion, C. elegans cultured in a defined axenic medium is a suitable and rapid model for studies on B12 deficiency.

History

Journal

Journal of nutrition and intermediary metabolism

Volume

13

Pagination

20-25

Location

Amsterdam, The Netherlands

Open access

  • Yes

eISSN

2352-3859

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2018, The Authors

Publisher

Elsevier