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Regulation of metabolic transcriptional co-activators and transcription factors with acute exercise

journal contribution
posted on 01.06.2005, 00:00 authored by Aaron RussellAaron Russell, M Hesselink, Sing Lo, P Schrauwen
Endurance exercise improves insulin sensitivity and increases fat oxidation, which are partly facilitated by the induction of metabolic transcription factors. Next to exercise, increased levels of FFA's also increase the gene expression of transcription factors, hence making it difficult to discern the effects from contractile signals produced during exercise, from those produced by increased circulatory FFA's. We aimed to investigate, in human skeletal muscle, whether acute exercise affects gene expression of metabolic transcriptional co-activators and transcription factors, including PGC-1α, PRC, PPARα, β/δ, and γ and RXR, SREBP-1c and FKHR, and to discern the effect of exercise per se from those of elevated levels of FFA. Two hours of endurance exercise was performed either in the fasted state, or following carbohydrate ingestion prior to and during exercise, thereby blunting the fasting-induced increase in FA availability and oxidation. Of the genes measured, PGC-1α and PRC mRNA increased immediately after, while PPARβ/δ and FKHR mRNA increased 1–4 h after exercise, irrespective of the increases in FFA's. Our results suggest that the induction in vivo of metabolic transcription factors implicated in mitochondrial biogenesis are under the control of inherent signals, (PGC-1α, PRC), while those implicated in substrate selection are under the control of associated signals (PPARβ/δ, FKHR) stimulated from the contracting skeletal muscle that are independent of circulating FFA levels.

History

Journal

The FASEB journal : official publication of the Federation of American Societies for experimental biology

Volume

19

Issue

8

Pagination

986 - 988

Publisher

Federation of American Societies for Experimental Biology

Location

Bethesda, Md.

ISSN

0892-6638

eISSN

1530-6860

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2007, FASEB