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Relationships of nicotianamine and other amino acids with nickel, zinc and iron in Thlaspi hyperaccumulators
journal contribution
posted on 2007-12-01, 00:00 authored by Damien CallahanDamien Callahan, S D Kolev, R A J O’Hair, D E Salt, A J M BakerExperimental evidence suggests that nicotianamine (NA) is involved in the complexation of metal ions in some metal-hyperaccumulating plants.
Closely-related nickel (Ni)- and zinc (Zn)-hyperaccumulating species were studied to determine whether a correlation exists between the Ni and Zn concentrations and NA in foliar tissues.
A liquid chromatography–mass spectrometry (LC-MS) procedure was developed to quantify the NA and amino acid contents using the derivatizing agent 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. A strong correlation emerged between Ni and NA, but not between Zn and NA. Concentrations of NA and l-histidine (His) also increased in response to higher Ni concentrations in the hydroponic solution supplied to a serpentine population of Thlaspi caerulescens. An inversely proportional correlation was found between the iron (Fe) and Ni concentrations in the leaves. Correlations were also found between Zn and asparagine.
The results obtained in this study suggest that NA is involved in hyperaccumulation of Ni but not Zn. The inverse proportionality between the Ni and Fe concentrations in the leaf may suggest that Ni and Fe compete for complexation to NA.
Closely-related nickel (Ni)- and zinc (Zn)-hyperaccumulating species were studied to determine whether a correlation exists between the Ni and Zn concentrations and NA in foliar tissues.
A liquid chromatography–mass spectrometry (LC-MS) procedure was developed to quantify the NA and amino acid contents using the derivatizing agent 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. A strong correlation emerged between Ni and NA, but not between Zn and NA. Concentrations of NA and l-histidine (His) also increased in response to higher Ni concentrations in the hydroponic solution supplied to a serpentine population of Thlaspi caerulescens. An inversely proportional correlation was found between the iron (Fe) and Ni concentrations in the leaves. Correlations were also found between Zn and asparagine.
The results obtained in this study suggest that NA is involved in hyperaccumulation of Ni but not Zn. The inverse proportionality between the Ni and Fe concentrations in the leaf may suggest that Ni and Fe compete for complexation to NA.
History
Journal
New phytologistVolume
176Issue
4Pagination
836 - 848Publisher
WileyLocation
London, Eng.Publisher DOI
ISSN
0028-646XeISSN
1469-8137Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2007, WileyUsage metrics
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No categories selectedKeywords
amino acidhyperaccumulatorliquid chromatography–mass spectrometry (LC-MS)nickelnicotianamineScience & TechnologyLife Sciences & BiomedicinePlant Sciencesliquid chromatography-mass spectrometry (LC-MS)ThlaspiTOMATO MUTANT CHLORONERVANORMALIZING FACTORMETAL TRANSPORTWILD-TYPECAERULESCENSTOLERANCEPLANTACCUMULATIONBRASSICACEAECOMPLEXATION
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