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Renal C-type natriuretic peptide and natriuretic peptide receptor B mRNA expression are affected by water deprivation in the Spinifex Hopping mouse
journal contributionposted on 2003-01-01, 00:00 authored by Rachel Heimeier, John DonaldJohn Donald
This study investigated the effect of water deprivation on the expression of C-type natriuretic peptide (CNP) and natriuretic peptide receptor B (NPR-B) mRNA, and the ability of NPR-B to generate cGMP in the Spinifex Hopping mouse, Notomys alexis. This rodent is a native of central and western Australia that is well adapted to survive in arid environments. Initially, CNP and NPR-B cDNAs (partial for NPR-B) were cloned and sequenced, and were shown to have high homology with those of rat and mouse. RT-PCR analysis showed CNP mRNA expression in the kidney, proximal and distal colon and small intestine, whilst NPR-B mRNA expression was found in the kidney, proximal and distal colon and the atria. Using a semi-quantitative multiplex PCR technique, the expression of renal CNP and NPR-B mRNA was determined in 7- and 14-day water-deprived hopping mice, in parallel with control hopping mice (access to water). Water deprivation significantly decreased the relative levels of CNP and NPR-B mRNA expression in both the 7- and 14-day water-deprived hopping mice, when compared to control hopping mice. In contrast, the ability of CNP to stimulate cGMP production was significantly increased after 14 days of water deprivation. This study shows that alterations in the renal CNP/NPR-B system may be an important physiological adjustment when water is scarce.
JournalComparative biochemistry and physiology. Part A, Molecular & integrative physiology
Pagination565 - 575
LocationNew York, N.Y.
NotesAvailable online 26 August 2003.
Publication classificationC1 Refereed article in a scholarly journal
Copyright notice2003, Elsevier Inc.
C-type natriuretic peptidenatriuretic peptide receptor BmRNA expressionwater deprivationnotomys alexiskidneyScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyPhysiologyZoologySEQUENCE-ANALYSISNOTOMYS-ALEXISDESERT RODENTSRATPRECURSORSYSTEMCNPLOCALIZATIONVASOPRESSINCLONINGPhysiologyZoology